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How to use LR ELISA Kit to detect leukoregulin
Topic Started: Tuesday Jan 21 2014, 09:06 AM (21 Views)
cusabiozdy

Leukoregulin(LR) is a 50-kDa T lymphocyte-derived protein. It influences the synthesis of enzyme, stromelysin-1, collagen, and hyaluronan in human fibroblasts and is so a determinant of living thing matrix economy.
The LR ccl11 elisa kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to LR. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for LR and Avidin-Horseradish Peroxidase conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain LR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow.
The impact of leukoregulin on the expression of urokinase matter kind one(PAI-1) in human orbital and dermal fibroblasts. The cytokine upregulated 35S-labeled PAI-1 supermolecule expression in orbital fibroblasts in dose-dependent manner. Leukoregulin down PAI-1 supermolecule levels dramatically in skin fibroblasts from the paries. With relevance PAI-1 expression, it might seem that the anatomic web site of origin of fibroblasts could be a crucial determinant of the cellular response to leukoregulin.
Leukoregulin will elicit in orbital fibroblasts a dramatic increase within the rate of hyaluronan synthesis. In distinction, hyaluronan production in dermal fibroblasts is altered, however to a significantly lesser degree. This protein action was additionally attenuated by glucocorticoids. Expression of urokinase matter kind one informational RNA and supermolecule is up-regulated by leukoregulin in orbital fibroblasts to levels that area unit 75-fold on top of baseline. In distinction, urokinase matter kind one synthesis in dermal fibroblasts from the paries is down-regulated. it might seem that orbital fibroblasts exhibit a novel status to multiple actions of leukoregulin.
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